BI21X Laboratory Safety Protocols

DO NOT:

  • Eat, drink, or handle unnecessary equipment (cell phones, clickers, etc.) in the laboratory.
  • Taste, touch, or smell any reagents unless directed to do so by your instructor. When smelling chemicals or gases, use a wafting motion to direct the odor toward your nose.
  • Discard insoluble materials in the sink.
  • Pour chemicals back into containers.
  • Move a lit flame.
  • Operate any laboratory equipment until you have been instructed in its use.
  • Engage in horseplay, pranks or other acts of mischief or disrespect for others.
  • Perform unauthorized experiments.

DO:

  • Assume that all reagents are poisonous and act accordingly.  If you come into contact with any reagent, wash immediately with water.  Material safety data sheets (MSDS) are available at the front of the lab.
  • Dispose of chemicals as instructed.
  • Keep your work area neat, clean, and organized. Store backpacks out of the way.
  • Become familiar with the experiments you will be doing before coming to the laboratory.
  • Completely follow the procedures set forth by the laboratory instructor. Breakage fees due to misuse will be charged to the student responsible for any broken equipment.
  • Be alert and aware of what is going on around you and always proceed with caution.
  • Know the location of emergency equipment, such as a first aid kit, eyewash bottle, fire extinguisher, and telephone. Report all accidents to your laboratory instructor immediately.
  • Exercise great caution when using heat. Handle hot glassware or labware with a clamp, tongs or hot pad.
  • Keep combustible materials away from flame.
  • Use extra care when working with scalpels, knives, glass tubing or other sharp-edged objects.
  • Discard needles, razor blades, scalpel blades and other such items only in the RED "Sharps" containers.
  • Discard cracked or broken microscope slides and other glass only in the broken glass containers. Never handle broken glass with your bare hands, use a dust pan and broom located under the sink.
  • Clean up spills wearing gloves and disposed of the gloves and cleaning materials appropriately.
  • Use proper ventilation and hoods when instructed.
  • Know the correct procedure for mixing acid solutions. Always add the acid slowly to the water. Never add water to a large amount of acid.
  • Wear clothing that, if damaged, would not be a serious loss, since some chemicals may damage fabrics.
  • Ideally, wear appropriate shoes (closed toe) and clothing (long pants) as protection against broken glass or spillage.
  • Report accidents, injuries, and any condition that appears unsafe or hazardous to the laboratory instructor.
  • Wash hands with soap and wipe down tables after the lab, and use proper procedures when handling bacteria, protozoans, and fungi. Notify your teacher before you begin the lab of any health problems you have which may have compromised your immune system.
  • Clean your work area and glassware and return materials to the proper place before leaving the laboratory.